Viscoelastic properties and dynamics of porcine gastric mucin.

نویسندگان

  • Jonathan Celli
  • Brian Gregor
  • Bradley Turner
  • Nezam H Afdhal
  • Rama Bansil
  • Shyamsunder Erramilli
چکیده

Gastric mucin is a glycoprotein known to undergo a pH-dependent sol-gel transition that is crucial to the protective function of the gastric mucus layer in mammalian stomachs. We present microscope-based dynamic light scattering data on porcine gastric mucin at pH 6 (solution) and pH 2 (gel) with and without the presence of tracer particles. The data provide a measurement of the microscale viscosity and the shear elastic modulus as well as an estimate of the mesh size of the gel formed at pH 2. We observe that the microscale viscosity in the gel is about 100-fold lower than its macroscopic viscosity, suggesting that large pores open up in the gel reducing frictional effects. The data presented here help to characterize physiologically relevant viscoelastic properties of an important biological macromolecule and may also serve to shed light on diffusive motion of small particles in the complex heterogeneous environment of a polymer gel network.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of acid modification of porcine mucin on its drug release and skin permeation properties in insulin transdermal patches

The study aimed at determining the effect of acid modified porcine mucin powder on the release and permeation of insulin in transdermal patches. Various batches of insulin patches were prepared by solvent casting method using polysorbate 80 as emulsifying agent and acid treated and untreated mucin powders as base. The patches were evaluated for their physical properties, folding endurance, moi...

متن کامل

Rheology of gastric mucin exhibits a pH-dependent sol-gel transition.

Gastric mucin, a high molecular weight glycoprotein, is responsible for providing the gel-forming properties and protective function of the gastric mucus layer. Bulk rheology measurements in the linear viscoelastic regime show that gastric mucin undergoes a pH-dependent sol-gel transition from a viscoelastic solution at neutral pH to a soft viscoelastic gel in acidic conditions, with the transi...

متن کامل

Helicobacter pylori moves through mucus by reducing mucin viscoelasticity.

The ulcer-causing gastric pathogen Helicobacter pylori is the only bacterium known to colonize the harsh acidic environment of the human stomach. H. pylori survives in acidic conditions by producing urease, which catalyzes hydrolysis of urea to yield ammonia thus elevating the pH of its environment. However, the manner in which H. pylori is able to swim through the viscoelastic mucus gel that c...

متن کامل

Molecular structure and rheological properties of short-side-chain heavily glycosylated porcine stomach mucin.

The current accepted model for high-molecular-weight gastric mucins of the MUC family is that they adopt a polydisperse coil conformation in bulk solutions. We develop this model using well-characterized highly purified porcine gastric mucin Orthana that is genetically close to the human MUC6 type. It has short side chains and low levels of sialic acid residues and includes minute amounts of cy...

متن کامل

Development and Rheological Profiling of Biosimilar Mucus

This article presents the development of a biosimilar mucus mixture for assessment of the effect of mucus on drug absorption. The biosimilar mucus was optimized to represent a rheological profile similar to that of porcine intestinal mucus. This was obtained with a mixture of mucin, polyacrylic acid, lipids and BSA. INTRODUCTION The viscoelastic mucus layers covering the epithelial surfaces are...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Biomacromolecules

دوره 6 3  شماره 

صفحات  -

تاریخ انتشار 2005